Protocol for Sampling Wheat or Barley Spikes Symptomatic with Fusarium head blight
Thank you for collaborating on this study of FHB-symptomatic wheat and barley spikes! The purpose is to determine the occurrence of several Fusarium species and their mycotoxins in head-blighted spikes from commercial grain fields.
For each field, please collect into:
1. Paper bag #1: 60 symptomatic spikes from locations in the field as widely separated as is practical. A suggested sampling pattern is shown below.
2. Paper bag #2: Six small pieces of debris from the previous crop, one piece of debris per each of 6 passes in the field. (If this part is too time-consuming, please omit.)
For each field, please record:
1. Nearest street address: _________________________________________________
2. GPS Coordinates: _________________________________
3. Estimated percentage of all spikes that have FHB symptoms (e.g., 50-100%, 30-49%, 20-29% or <20%): _________
4. Average severity on a blighted spike (what % of a symptomatic spike is affected, on average): _________
5. (If possible) Name and phone number of field manager or farm owner:
6. If you are able (if not, we will make phone calls):
a. estimated date of heading or flowering: ____________________
b. variety _________________________
c. tillage history __________________________________________
d. previous crop __________________________________________
Please send samples to: Briana Whitaker, USDA-ARS, 1815 N University St, Peoria, IL 61604. Questions: 336-269-1063 (cell)
Suggested sampling pattern: start at one corner of the field. Move along the row direction, collecting about 10 spikes in each of 6 passes across the field, as shown in the figure to the right.